human liver hepatocellular carcinoma cell line hepg2  (ATCC)

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: Fluorescent and visible light microscopy images of K562 (rows 1 and 2) and HepG2 (rows 3 and 4) cells incubated with culture medium, free DOX, DOX/HEP-DEX, and DOX/FA-HEP-DEX micelles after 4 h incubation. DOX, Doxorubicin; FA, folic acid; HEP, heparin; DEX, dexamethasone.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: Light Microscopy, Incubation

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: The cellular uptake percentage of free DOX, DOX/HEP-DEX, and DOX/FA-HEP-DEX micelles after 4 h incubation in K562 and HepG2 cells. DOX, Doxorubicin; FA, folic acid; HEP, heparin; DEX, dexamethasone.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: Incubation

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: In vitro cytotoxicity of free DOX, DOX-loaded micelles, and blank micelles evaluated against (A) K562 cells after 48 h incubation, (B) K562 cells after 72 h incubation, (C) HepG2 cells after 48 h incubation, and (D) HepG2 cells after 72 h incubation. Data are plotted as the mean ± SD, n = 3. DOX, Doxorubicin; FA, folic acid; HEP, heparin; DEX, dexamethasone.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: In Vitro, Incubation

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: IC 50 values of DOX from free DOX, DOX/FA-HEP-DEX and DOX/HEP-DEX micelles after 48 h and 72 h exposure to K562 and HepG2 cells. Data represent mean ± SD, n = 3. ** P < 0.01 and *** P < 0.001 represent significant differences in comparison with free DOX; ## P < 0.01 versus DOX/HEP-DEX.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: Comparison

Journal: Nature Communications

Article Title: Methylcobalamin protects against liver failure via engaging gasdermin E

doi: 10.1038/s41467-024-54826-6

Figure Lengend Snippet: a Procedure of terbium (Tb 3+ )/dipicolinic acid (DPA) fluorescence liposome leakage assay. b Percentage of inhibition on 829 compounds, assayed at 100 μM. Cutoff was 70% inhibition. Dimethyl fumarate (DMF) was used as a positive control. Each point is an individual inhibitor ( n = 1). c LDH release assay for cytotoxicity of HepG2 cells pretreated with 9 screening hits at 100 μM before challenged with TNFα/cycloheximide (CHX) for 16 h to induce GSDME-mediated pyroptosis. d Chemical structure of compound 19A11 (methylcobalamin, MeCbl). e 25 ~ 100 μM MeCbl or DMF were treated with 0.2 μM recombinant active-caspase-3 for activity assay. Z-DEVD-FMK was used as positive control. f MST measurements of the binding of 0.2 μM recombinant caspase-3, GSDME, GSDMD with MeCbl or positive control. Values of K d are listed in the graphs. Data are mean ± SEM ( n = 3) of at least two independent experiments for bar charts. Analysis was done using one-way ANOVA ( c , e ). p < 0.05 is considered significant and p values are provided in graphs, n.s = nonsignificant. All groups were compared to indicated vehicle. Source data are provided as Source Data file.

Article Snippet: Liver hepatocellular cell line HepG2 (ATCC, HB-8065, male) and human embryonic kidney cell line HEK293T (ATCC, CRL-3216, female) were purchased from ATCC (Virginia, USA).

Techniques: Fluorescence, Inhibition, Positive Control, Lactate Dehydrogenase Assay, Recombinant, Activity Assay, Binding Assay

Journal: Nature Communications

Article Title: Methylcobalamin protects against liver failure via engaging gasdermin E

doi: 10.1038/s41467-024-54826-6

Figure Lengend Snippet: a , c , d , e HepG2 were pretreated with indicated concentrations of each compound for 2 h before adding 200 μM deoxycholic acid (DCA) for 4 h, the cytotoxicity of HepG2 cells was determined by LDH release assay ( a ). Pyroptosis was measured by SYTOX green uptake in the presence of 20 μM MeCbl or DMF ( c ). Representative immunoblotting analysis of caspase-3 and GSDME in HepG2 ( d ), and caspase-3 activity was assessed with substrate Ac-DEVD-pNA ( e ). b Bone marrow derived macrophages (BMDMs) were pretreated with indicated concentrations of MeCbl for 2 h before challenged with LPS transfection by Fugene HD for GSDMD-mediated pyroptosis. Cytotoxicity was determined by LDH release assay. f Changes in HepG2 cell morphology were observed with a microscope (scale bar= 10 μm). g Representative images of GSDME localization in HepG2 cells treated as indicated by confocal microscopy (scale bar= 5 μm). h , i Mice primary hepatocytes were pretreated with indicated concentrations of each compound for 2 h before challenged with 40 nM perforin (PFR) and 0.5 μM GzmB simultaneously for 24 h to activate GSDME-mediated pyroptosis, the cytotoxicity of hepatocytes was determined by LDH release assay ( h ). Pyroptosis was measured by SYTOX green uptake in the presence of 20 μM MeCbl or DMF ( i ). j Effect of MeCbl on PFR+GzmB-induced LDH release in hepatocytes pretreated with Z-DEVD-FMK (20 μM). k , m WT, Gsdmd -/- , Gsdme -/- hepatocytes were pretreated with indicated inhibitors for 2 h before challenged with Fugene HD/LPS for 16 h ( k ) or 200 μM DCA for 4 h ( m ), cytotoxicity was determined by LDH release assay. l , n Representative immunoblotting analysis of GSDMD and GSDME in hepatocytes. Data are mean ± SEM ( n = 3) of at least two independent experiments for bar and line charts. Blots and micrographs are representative of three independent experiments. Analysis was done using one-way ANOVA ( e ) or two-way ANOVA ( a – c , h – k , m ). p < 0.05 is considered significant to the indicated group and p values are provided in graphs, n.s = nonsignificant compared to vehicle ( e ) or indicated group ( k , m ). Source data are provided as Source Data file.

Article Snippet: Liver hepatocellular cell line HepG2 (ATCC, HB-8065, male) and human embryonic kidney cell line HEK293T (ATCC, CRL-3216, female) were purchased from ATCC (Virginia, USA).

Techniques: Lactate Dehydrogenase Assay, Western Blot, Activity Assay, Derivative Assay, Transfection, Microscopy, Confocal Microscopy

Journal: BMC complementary medicine and therapies

Article Title: Effects of essential oil of Origanum onites and its major component carvacrol on the expression of toxicity pathway genes in HepG2 cells.

doi: 10.1186/s12906-024-04571-6

Figure Lengend Snippet: Fig. 2 Inhibition effects of DNA synthesis from the EOOO. (0.02–0.2 µg/mL) (A) and CV (7.5–105 µg/mL) (B) on the growth of HepG2 cells after 48 h. The EOOO and CV induced inhibition of DNA syntheses were in a concentration-dependent manner by the method BrdU incorporation assay. All values are expressed as mean ± SD at least three separate experiments performed in quadruplicate. Differences were considered significant compared to the control group from *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

Article Snippet: A human liver hepatocellular carcinoma cell line HepG2 purchased from DSMZ (Braunschweig, Germany) was cultured in DMEM (Dulbecco Modified Eagle Medium) (Sigma) supplemented with 10% FBS (fetal bovine serum) (PAA Lab. GmbH, Les Mureaux, France), penicillin/ streptomycin at 100 units/ml and 2 mM L-glutamine as adherent monolayers.

Techniques: Inhibition, DNA Synthesis, Concentration Assay, BrdU Incorporation Assay, Control

Journal: BMC complementary medicine and therapies

Article Title: Effects of essential oil of Origanum onites and its major component carvacrol on the expression of toxicity pathway genes in HepG2 cells.

doi: 10.1186/s12906-024-04571-6

Figure Lengend Snippet: Fig. 3 Effects of the EOOO (A) (0.08 and 0.09 µg/mL) and (B) CV (45 and 75 µg/mL) on viability of HepG2 cells incubated for 24, 48, 72 and 96 h. Each value is the mean ± S.D. of three separate experiments performed in quadruplicate. Differences were considered significant compared to the control group from *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

Article Snippet: A human liver hepatocellular carcinoma cell line HepG2 purchased from DSMZ (Braunschweig, Germany) was cultured in DMEM (Dulbecco Modified Eagle Medium) (Sigma) supplemented with 10% FBS (fetal bovine serum) (PAA Lab. GmbH, Les Mureaux, France), penicillin/ streptomycin at 100 units/ml and 2 mM L-glutamine as adherent monolayers.

Techniques: Incubation, Control